Rat ADRb3 (Adrenergic Receptor Beta 3) ELISA Kit - ELK6915

Rat ADRb3 (Adrenergic Receptor Beta 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6915-48, ELK6915-96, ELK6915-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: BETA3AR; B3AR; Beta-3 adrenoreceptor

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Endocrinology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADRb3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADRb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADRb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADRb3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P26255

Research Use Only