Rat FADS1 (Fatty Acid Desaturase 1) ELISA Kit - ELK6210

Rat FADS1 (Fatty Acid Desaturase 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6210-48, ELK6210-96, ELK6210-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: D5D; FADS6; FADSD5; LLCDL1; TU12; Delta-5 Desaturase; Delta (5) fatty acid desaturase

Assay Type: Sandwich

Sensitivity: 24.6 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Metabolic pathway;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FADS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FADS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FADS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FADS1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q920R3

Research Use Only