Rat GAL (Galanin) ELISA Kit - ELK5651

Rat GAL (Galanin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5651-48, ELK5651-96, ELK5651-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide

Assay Type: Competitive Inhibition

Sensitivity: 4.94 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Endocrinology; Reproductive science; Neuroscience; Hepatology; Gastroenterology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GAL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GAL in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P10683

Research Use Only