Rat GR (Glutathione Reductase) ELISA Kit - ELK6025

Rat GR (Glutathione Reductase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6025-48, ELK6025-96, ELK6025-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GSR; GLUR; GRD1; Glutathione reductase, mitochondrial

Assay Type: Sandwich

Sensitivity: 0.064 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GR in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P70619

Research Use Only