Rat HJV (Hemojuvelin) ELISA Kit - ELK6367

Rat HJV (Hemojuvelin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6367-48, ELK6367-96, ELK6367-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HFE2; HFE2A; HJV; JH; RGMC; hemochromatosis type 2 (Juvenile); Haemojuvelin; RGM domain family member C

Assay Type: Sandwich

Sensitivity: 14.6 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway; Infection immunity; Hematology; Nutrition metabolism; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HJV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HJV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HJV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HJV in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q8N7M5

Research Use Only