Rat INSL3 (Insulin Like Protein 3) ELISA Kit - ELK6400

Rat INSL3 (Insulin Like Protein 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6400-48, ELK6400-96, ELK6400-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: RLNL; RLF; Relaxin-Like Factor,Leydig Cell; Leydig insulin-like peptide

Assay Type: Competitive Inhibition

Sensitivity: 62 pg/mL

Standard: 15000 pg/mL

Detection Range: 234.38-15000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Endocrinology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat INSL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat INSL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat INSL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9WUK0

Research Use Only