Rat MELK (Maternal Embryonic Leucine Zipper Kinase) ELISA Kit - ELK6957

Rat MELK (Maternal Embryonic Leucine Zipper Kinase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6957-48, ELK6957-96, ELK6957-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: hPK38; Protein kinase Eg3; Tyrosine-protein kinase MELK

Assay Type: Sandwich

Sensitivity: 0.066 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MELK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MELK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MELK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MELK in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: D4A6T6

Research Use Only