Rat NT (Neurotensin) ELISA Kit - ELK6069

Rat NT (Neurotensin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6069-48, ELK6069-96, ELK6069-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NTS; NTS1; NmN-125; NN; Tail peptide Neuromedin N

Assay Type: Competitive Inhibition

Sensitivity: 14.5 pg/mL

Standard: 3000 pg/mL

Detection Range: 46.88-3000 pg/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Reproductive science; Neuroscience; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P20068

Research Use Only