Rat Pg (Progesterone) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-OSEL-R0007-24, E-OSEL-R0007-48, E-OSEL-R0007-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: Pg
UNIProt ID: ???
Target Synonyms: Progesterone; Pg;
Research Areas: Signal Transduction
Target Species: Rat
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Rat Pg concentrations in serum, plasma.
Tested Sample Types: Serum, Plasma
Type: Competitive
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 7.01 pg/mL
Detection Range: 15.63-1000 pg/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Pg are added to the micro ELISA plate wells. Rat Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Rat Pg in samples. No significant cross-reactivity or interference between Rat Pg and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag