Rat PPARa (Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit - ELK4576

Rat PPARa (Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4576-48, ELK4576-96, ELK4576-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NR1C1; PPAR-A; HPPAR; Nuclear Receptor Subfamily 1 Group C Member 1

Assay Type: Sandwich

Sensitivity: 0.11 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Tumor immunity; Developmental science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPARa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPARa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPARa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PPARa in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P37230

Research Use Only