Rat PRKAb1 (Protein Kinase, AMP Activated Beta 1) ELISA Kit - ELK5668

Rat PRKAb1 (Protein Kinase, AMP Activated Beta 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5668-48, ELK5668-96, ELK5668-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: AMPK; HAMPKb; 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit; Adenosine Monophosphate Activated Protein Kinase (AMPK)

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Metabolic pathway;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRKAb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRKAb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRKAb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRKAb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P80386

Research Use Only