Rat SGPP1 (Sphingosine-1-Phosphate Phosphatase 1) ELISA Kit - ELK6976

Rat SGPP1 (Sphingosine-1-Phosphate Phosphatase 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6976-48, ELK6976-96, ELK6976-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SPPase1

Assay Type: Sandwich

Sensitivity: 0.06 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SGPP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SGPP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SGPP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SGPP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q99P55

Research Use Only