Rat TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit - ELK5664

Rat TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5664-48, ELK5664-96, ELK5664-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HITF; ITF3; TFI; HP1.B

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Tumor immunity; Gastroenterology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFF3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFF3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q03191

Research Use Only