Product Description

Rb (phospho-S807) Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9862M-50, BS9862M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06400

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Background: The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Rb protein only when phosphorylated at Ser807.

Bioworld Molecular Weight: ~ 106 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rb (phospho-S807) Rabbit mAb at 1:1000 dilution
Lane 1: A549 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Rb(S807) antibody. Counter stained with hematoxylin., ICC staining Phospho-Rb(S807) in A549 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Phosphorylation