Product Description

RbAP48 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91160-50, BS91160-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q09028(Human) Q60972(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC IP,

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: In the intact cell, DNA is closely associated with histones and other nuclear proteins to form chromatin.The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation, and an increased accessiblity of DNA to transcription factors. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The retinoblastoma binding proteins RbAp46 and RbAp48 have been identified as histone binding proteins, and they are components of the histone deacetylase complex.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: RbAP48 polyclonal antibody detects endogenous levels of RbAP48 protein.

Bioworld Molecular Weight: 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: Hela
Lane 3: Rat brain
Lane 4: Mouse testis, ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4, RBBP4, RBAP48, CAF I p48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Chromatin assembly factor/CAF 1 p48 subunit, Histone-binding protein RBBP4, MSI1 protein homolog, Nucleosome-remodeling factor subunit RBAP48, NURF55, RB binding protein 4 chromatin remodeling factor, RbAp 48, RBAP48, RBBP-4, RBBP4, RBBP4, Retinoblastoma binding protein 4, Retinoblastoma binding protein p48, Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified