RBBP5 (1C9) Monoclonal Antibody-MB0173

RBBP5 (1C9) Monoclonal Antibody

Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: MB0173-50, MB0173-100

Swiss-Prot: Q15291

Host: Mouse

RBBP5 (1C9) Monoclonal Antibody-MB0173

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB: 1:1000
IF: 1:50~200

Background: This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: This antibody detects endogenous levels of RBBP5 and does not cross-react with related proteins.

Bioworld Molecular Weight: Predicted band size:59KDa
Observed band size:70KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blotDetection of RBBP5 in C2C12,THP-1,PC12 and 3T3 cellLysates using RBBP5 mouse mAb (1:1000 diluted) ., Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-RBBP5 mouse mAb (dilution 1:100) . DAPI was used to stain nucleus (blue) .

Alternative Name: RBBP 5; RBBP-5; RBBP5; RBBP5_HUMAN; RBQ 3; RBQ3; Retinoblastoma binding protein 5; Retinoblastoma binding protein RBQ3; Retinoblastoma binding protein5; Retinoblastoma-binding protein 5; Retinoblastoma-binding protein RBQ-3; SWD1; SWD1, Set1c WD40 repeat protein, homolog.

Immunogen: Purified recombinant human RBBP5 protein fragments expressed in E.coli.

Modification: Unmodified

Conjugate: Unconjugated