RBBP5 Monoclonal Antibody-MB66603

RBBP5 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66603-50, MB66603-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q15291

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background: The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator. While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4. These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity. MLL1 and MLL2 complexes contain the additional protein subunit, menin. Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4. MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of RBBP5 protein.

Bioworld Molecular Weight: ~ 67 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RBBP5 expression in C2C12 (A), THP1 (B), PC12 (C), NIH3T3 (D) whole cell lysates., Immunofluorescent analysis of RBBP5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Alternative Name: RBQ3; Retinoblastoma-binding protein 5; RBBP-5; Retinoblastoma-binding protein RBQ-3

Immunogen: Purified recombinant human RBBP5 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified