RBBP6 Polyclonal Antibody-BS8619

RBBP6 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS8619-50, BS8619-100

Swiss-Prot: Q7Z6E9

Host: Rabbit

RBBP6 Polyclonal Antibody-BS8619

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: IF

All Applications: IF 1:50 - 1:200

Background: Rb protein and p53 are both cell cycle checkpoint components. Evidence suggests that p53 plays a role in regulating the phosphorylation of Rb by inducing p21 transcription, thus preventing Rb phosphorylation at the G1 to S transition. Protein-protein interactions seem to be central in p53 cellular activities, as previously demonstrated with MDM2 and SV40 large T antigen. Two novel proteins have been identified by their abilities to bind to p53 and/ or Rb. Human RBQ-1 (also designated RBBP6) has been cloned as a novel protein that binds to the retinoblastoma (Rb) gene product. A related mouse protein, P2P-R, also designated PACT (for p53 associated cellular protein-testis derived), has been shown to bind to both Rb and p53. Recombinant P2P-R binds to wildtype p53 but not to mutant p53, and it can interfere with p53 specific DNA binding. RBQ-1 may be a truncated human form of the P2P-R protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: RBBP6 polyclonal antibody detects endogenous levels of RBBP6 protein.

Bioworld Molecular Weight: ~ 160kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RBBP6 antibody., Immunofluorescence analysis of MCF7 cell using RBBP6 antibody. Blue: DAPI for nuclear staining.

Alternative Name: E3 ubiquitin-protein ligase RBBP6; EC=6.3.2.-; Proliferation potential-related protein; Protein P2P-R; Retinoblastoma-binding Q protein 1; RBQ-1; Retinoblastoma-binding protein 6; p53-associated cellular protein of testis; RBBP6; P2PR, PACT, RBQ1; My038

Immunogen: Recombinant full length Human RBBP6.

Modification: Unmodified

Conjugate: Unconjugated