RBP2 Polyclonal Antibody-BS8380

RBP2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8380-50, BS8380-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P50120

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200

Background: Rb (retinoblastoma protein) is a potent transcriptional regulator that is directly involved with events such as entry into cell division and formation of heterochromatin. RBP2 (Retinoblastoma-binding protein 2), also known as RBBP2, JARID1A (Jumonji/ARID domain-containing protein 1A) or KDM5A, is a nuclear protein that belongs to the JARID1 histone demethylase family. Expressed ubiquitously, RBP2 functions as a histone demethylase that, in conjunction with other proteins, binds directly to the viral-binding domain of Rb, thereby regulating Rb-mediated cell proliferation events. In addition, RBP2 can bind to the Rb-interacting protein rhombotin-2 (LMO2) and, through this interaction, can indirectly modulate Rb activity. Via its demethylase activity, RBP2 can remove methyl residues from Histone H3, thus playing a crucial role in the histone code. RBP2 contains one ARID domain, three PHD-type zinc fingers, one JMJN domain and one JMJC domain through which it conveys its enzymatic activity. Multiple isoforms of RBP2 exist due to alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RBP2 polyclonal antibody detects endogenous levels of RBP2 protein.

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cells, using RBP2 antibody., Immunohistochemistry of paraffin-embedded human liver injury using RBP2 antibody at dilution of 1:100 (x40 lens)., Immunohistochemistry of paraffin-embedded human gastric using RBP2 antibody at dilution of 1:100 (x40 lens).

Alternative Name: Retinol-binding protein 2; Cellular retinol-binding protein II; CRBP-II; RBP2; CRBP2

Immunogen: Recombinant full length Human RBP2.

Conjugate: Unconjugated

Modification: Unmodified