Product Description

RCC1 Monoclonal Antibody

Catalogue Numbers: MB66914-50, MB66914-100

Sizes: 50µl, 100µl

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P18754

Host: Mouse

Reactivity: Human, Mouse

Applications: WB

All Applications: WB (1/1000 - 1/2000)

Background: The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport . Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor activity, which catalyzes the exchange of bound GDP for GTP. Regulator of chromatin condensation 1 is the only known RanGEF . RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally. Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation and methylation. RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis . In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclin B/cdc2 . This phosphorylation may play a role in RCC1 interaction with chromatin and RCC1 RanGEF activity .

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of RCC1 protein.

Extra Notes: Western blot analysis of RCC1 expression in Hela (A), HL60 (B), COS7 (C), C2C12 (D) whole cell lysates.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: CHC1; Regulator of chromosome condensation; Cell cycle regulatory protein; Chromosome condensation protein 1

Immunogen: Recombinant fusion protein of human RCC1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified