Product Description

RCN1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78220-50, BS78220-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15293

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RCN1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of L929 cells using RCN1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using RCN1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: RCN1; HEL-S-84; PIG20; RCAL; RCN; reticulocalbin-1

Immunogen: Recombinant fusion protein of human RCN1(NP_002892.1).

Conjugate: Unconjugated

Modification: Unmodified