RDM1 Polyclonal Antibody-BS61252

RDM1 Polyclonal Antibody

Catalogue Numbers: BS61252-50, BS61252-100

Sizes: 50µl, 100µl

Alternative Name: RAD52 motif-containing protein 1; RAD52 homolog B; RDM1; RAD52B

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8NG50

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: RDM1 (RAD52motif-containing protein 1) is a 284 amino acid protein that is expressed in testis. RDM1 isinvolved in the cellular response to cisplatin, a drug commonly used in chemotherapy. Human RDM1 shares 71.1%and 51.2% amino acid identitywithmouse and chicken RDM1, respectively. RDM1 exists as 11 alternativelyspliced isoforms and only exon 3 iscommon to allvarants. Usage of two translationalstartsites, one in exon 1 and the other in intron 1, produces RDM1 isoformswith either long orshort N termini, and RDM1 isoforms have great diversityin their C termini as well. The RDM1 protein containstwomotifs: amotif found in Rad52, a protein that functions in DNA double-strand breaks and homologousrecombination, and an RNA recognitionmotif (RRM) that is not found in Rad52. The RDM1 gene contains seven coding exons, isconserved in chimpanzee,canine, bovine,mouse, rat, chicken and zebrafish, andmapsto human chromosome 17q12.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RDM1 polyclonal antibody detects endogenous levels of RDM1 protein.

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RDM1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human RDM1.

Conjugate: Unconjugated

Modification: Unmodified