Recombinant 2019-nCoV Envelope Protein (His Tag)
Size: 1mg
Catalogue Numbers: PKSR030488-1
Citations, Manuals and MSDS Available upon request.
Abbreviation: Envelope
Target Synonym: 2019-nCoV E protein; 2019-nCoV sM protein
UNIProt ID: P0DTC2
Target Species: SARS-CoV-2
Expression Host: E.coli
Fusion Tag: N-6His
Accession: QHD43418.1
Background: Coronavirus envelope (E) proteins are short (100 residues) polypeptides that contain at least one transmembrane (TM) domain and a cluster of 2-3 juxtamembrane cysteines. These proteins are involved in viral morphogenesis and tropism, and their absence leads in some cases to aberrant virions, or to viral attenuation. In common to other viroporins, coronavirus envelope proteins increase membrane permeability to ions, plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis. Activates the host NLRP3 inflammasome, leading to IL-1beta overproduction.
Sequence: Met1-Val75
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 200 mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 27.5 kDa
Observed MW: 22-25 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only