Recombinant 2019-nCoV Guanine-N7_meth Protein (His Tag) - PKSR030473

Recombinant 2019-nCoV Guanine-N7_meth Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSR030473-10, PKSR030473-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Guanine-N7_meth

Target Synonym: SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase

Target Species: SARS-CoV-2

Expression Host: E.coli

Fusion Tag: N-6His

Accession: YP_009725309.1

Background: The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). SARS-CoV 2 polyproteins incorporate 16 protein domains (nsps). The putative non-structural protein 2 (nsp2) of SARS-CoV plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development.

Sequence: Ala1-Gln527

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μM filtered solution of PBS, 10% Glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.9 kDa

Observed MW: 60 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only