Recombinant 2019-nCoV Methyltransferase / ME Protein (His Tag)
Size: 100μg
Catalogue Number: PKSR030513-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Methyltransferase; ME
Target Symptom: Methyltransferase; ME
Target Species: SARS-CoV-2
Expression Host: E.coli
Fusion Tag: C-6His
Accession: YP_009724390.1
Background: Coronavirus encodes the 2'-O-MTase（2'O Methyltransferase） that is composed of the catalytic subunit nsp16 and the stimulatory subunit nsp10 and plays an important role in virus genome replication and evasion from innate immunity during viral infection. Nonstructural protein 16 (NSP16) / viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting on the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral durgs development which can against SARS-CoV-2, SARS-CoV, MERS-CoV or other RNA and DNA viruses.
Sequence: Ser6799-Asn7096
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20 mM Tris 150 mM NaCl, pH 8.3.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 33.46 kDa.
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only