Recombinant Cynomolgus Interleukin-4 Receptor Subunit Alpha/IL-4 Rα Protein (His Tag) - PKSQ050053

Recombinant Cynomolgus Interleukin-4 Receptor Subunit Alpha/IL-4 Rα Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSQ050053-10, PKSQ050053-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: IL-4RA

Target Symptom: Interleukin-4 receptor subunit alpha; IL-4R-alpha; CD124; IL4-BP; Soluble IL-4R-alpha

Target Species: Cynomolgus macaques

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-His

UNIProt ID: G7Q0S7

Accession: G7Q0S7

Background: Interleukin-4 receptor subunit alpha(IL-4RA), alos known as Soluble IL-4 receptor subunit alpha, belongs to the type I cytokine receptor family and type 4 subfamily. It expressed in both Th1 and Th2 cells. It functions as receptor for both interleukin 4 and interleukin 13 and couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and chemokine and mucus production at sites of allergic inflammation. In certain cell types, IL-4RA can signal through activation of insulin receptor substrates, IRS1/IRS2. The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequently it recruits to the complex of the common gamma chain. In immune cells, IL-4RA creates a type I receptor. In non-immune cells, it forms a type II receptor with of IL13RA1. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor and interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP.

Activity: Immobilized Human IL-4(Cat: PKSH033456) at 10μg/ml(100 μl/well) can bind Human IL-4Ra-His. The ED50 of Human IL-4Ra-His is 7μg/mL.

Sequence: Met26-Arg232

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 24.6 kDa

Observed MW: 40 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only