Recombinant Dechloromonas aromatica Chlorite Dismutase Protein (His Tag) - PKSQ050054

Recombinant Dechloromonas aromatica Chlorite Dismutase Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSQ050054-10, PKSQ050054-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Chlorite O(2)-lyase; Chlorite Dismutase

Target Synonym: Chlorite dismutase; Chlorite O(2)-lyase; Daro_2580; Cld

UNIProt ID: Q47CX0

Target Species: Dechloromonas aromatica

Expression Host: E.coli

Fusion Tag: N-His

Accession: Q47CX0

Background: Chlorite dismutase (Cld) found in prokaryotic organisms, also known as Chlorite O2-lyase, is a b-type heme containing enzyme that catalyzes the reduction of chlorite into chloride plus dioxygen. The subunit of chlorite dismutase consists of a heme free N-terminal and a heme b containing C-terminal ferredoxin-like fold with high structural homology to the dye-decolorizing peroxidases (DyPs). The physiological role of Cld in prokaryote has been shown that some microorganisms can use perchlorate or chlorate as terminal electron acceptors for anaerobic respiration thereby producing chlorite that must be detoxified. This enzyme has gained attention because it can be used in the development of bioremediation processes, biosensors, and controlled dioxygen production.

Sequence: Met35-Asp282

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 0.5mM EDTA, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 31.3 kDa

Observed MW: 32 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only