Product Description

Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag)

Size: 100μg

Catalogue Number: PDEO100002-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: LacI

Target Synonym: Lactose operon repressor, LacI

UNIProt ID: P03023

Target Species: E.coli

Expression Host: E.coli

Fusion Tag: C-His

Accession: P03023

Background: Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.

Sequence: Met1-Gln360

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 39.4 kDa

Observed MW: 40 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only