Recombinant Endostatin, Human
Catalogue Numbers: BK0337-20, BK0337-100
Sizes: 20μg, 100μg
Source: P. pastoris
Molecular Weight: ~20.2 kDa, observed by reducing SDS-PAGE.
Purity: Greater than 97% by SDS-PAGE
Biological Activity: Determined by Endothelial Cell Tube Formation. The cells treated with 10μg/ml Endostatin showed significant anti-angiogenesis effect.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The sterile filtered solution was lyophilized with 17 mM citric-phosphate buffer, pH 6.2.
AA Sequence: AH SHRDFQPVLH LVALNSPLSG GMRGIRGADF QCFQQARAVG LAGTFRAFLS SRLQDLYSIV RRADRAAVPI VNLKDELLFP SWEALFSGSE GPLKPGARIF SFDGKDVLRH PTWPQKSVWH GSDPNGRRLT ESYCETWRTE APSATGQASS LLGGRLLGQS AASCHHAYIV LCIENSFMTA SK
Endotoxin: Endotoxin level is less than 0.1 ng per μg (1EU/μg) of Endostatin.
Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.
Storage: The lyophilized Endostatin is stable for a few weeks at room temperature, but best stored at -20°C. For the first use, reconstitute in water, aliquot and store at -20°C to avoid multiple freeze-thaws.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Endostatin is a cleaved product of the carboxyl-terminal domain of collagen XVIII. It functions as an anti-angiogenic cytokine that is expressed in various organs especially with high levels in liver, lung, and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant Endostatin expressed in yeast, whichcauses G1 arrest of endothelial cells. Endostatin treatment results in apoptosis of HUVE and HMVE cells. Recombinant human Endostatin is a 20.2 kDa protein consisting of 184 amino acid residues.