Recombinant FGF-10, Human
Catalogue Numbers: -10, -50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 19.3 kDa, observed by reducing SDS-PAGE.
Purity: > 95% as analyzed by SDS-PAGE.
Biological Activity: ED50 < 20 ng/mL, measured by a cell proliferation assay using 4 MBr-5 cells, corresponding to a specific activity of > 5.0 × 10ˆ4 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF
LKIEKNGK
VS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK
LYGSKEFNND CKLKERIE
EN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG
QKTRRKNTSA HFLPMVVHS
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/ml.
Storage: Lyophilized recombinant Human FGF-10 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human FGF-10 should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in the lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares homology with KGF and receptor binding to FGFR2-IIIb. However, while KGF induces proliferation and differentiation of various epithelial cells, FGF-10 promotes budding and branching morphogenesis during the multi-organ development via mesenchymal-epithelial cell interactions. FGF-10 is critical for lung and limb development, and is regulated by Shh during early development. Recombinant Human FGF-10 produced in E.coli is a single, non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhFGF-10 has a molecular mass of 19.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.