Recombinant FGF-4, Human -

Recombinant FGF-4, Human

Catalogue Numbers: -5, -25

Sizes: 5μg, 25μg

Source: Escherichia coli

Molecular Weight: 19.4kDa, observed by reducing SDS-PAGE.

Purity: > 95% by SDS-PAGE and HPLC analyses.

Biological Activity: ED50<0.5ng/ml, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >2.0× 10ˆ6 units/mg.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH7.5.

AA Sequence: MAPTAPNGT LEAELERRWE SLVALSLARL PVAAQPKEAA VQSGAGDYLL GIKRLRRLYC NVGIGFHLQA LPDGRIGGAH ADTRDSLLEL SPVERGVVSI FGVASRFFVA MSSKGKLYGS PFFTDECTFK EILLPNNYNA YESYKYPGMF IALSKNGKTK KGNRVSPTMK VTHFLPRL

Endotoxin: <0.2 EU/μg, determined by LAL method.

Reconstitution: Reconstituted in ddH2O at 100 μg/ml.

Storage: Lyophilized recombinant human Fibroblast Growth Factor-4 (rhFGF-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Description: Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis.Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E.coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques at GenScript, rhFGF-4 has a molecular mass of 19.4kDa as analyzed by reducing SDS-PAGE.