Recombinant FGF-6, Human
Catalogue Numbers: BK0043-10, BK0043-50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 18.8 kDa, observed by reducing SDS-PAGE.
Purity: > 95% as analyzed by SDS-PAGE and HPLC.
Biological Activity: ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence 1 µg/mL heparin, corresponding to a specific activity of > 4× 10ˆ5 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: MGTRANNTL LDSRGWGTLL SRSRAGLAGE IAGVNWESGY LVGIKRQRRL YCNVGIGFHL QVLPDGRISG THEENPYSLL EISt VERGVV SLFGVRSALF VAMNSKGRLY ATPSFQEECK FRETLLPNNY NAYESDLYQG TYIALSKYGR VKRGSKVSPI Mt VTHFLPRI
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/mL.
Storage: Lyophilized recombinant human Fibroblast Growth Factor-6 (rhFGF-6) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-6 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Fibroblast Growth Factor-6 (FGF-6) is a cytokine belonging to the heparin-binding FGF family, and is structurally related to other members of FGF family, particularly FGF-4. In vivo, FGF-6 exhibits an expression profile predominantly restricted tothe myogenic lineage, and it preferentially binds to two of the FGF receptors: FGFR1 and FGFR4. FGF-6 functions in muscle regeneration, myoblast proliferation and migration, and muscle differentiation in a dose-dependent manner. In vivo high concentration of recombinant FGF-6 up-regulates and down-regulates FGFR1 and FGFR4, respectively, as FGFR1 promotes the proliferation while FGFR4 promotes the differentiation in the muscle. Besides its dual function in muscle regeneration, FGF-6 may act as a regulator of bone metabolism as well.Recombinant human Fibroblast Growth Factor-6 (rhFGF-6) produced in E.coli is a single non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhFGF-6 has a molecular mass of 18.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.