Recombinant FGF-9, Human
Catalogue Numbers: BK0049-10, BK0049-50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 23.4 kDa, observed by reducing SDS-PAGE.
Purity: > 95% by SDS-PAGE and HPLC analyses.
Biological Activity: ED50 < 2.0 ng/ml , measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >5.0× 10ˆ5 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: MAPLGEVGN YFGVQDAVPF GNVPVLPVDS PVLLSDHLGQ SEAGGLPRGP AVTDLDHLKG ILRRRQLYCR TGFHLEIFPN GTIQGTRKDH SRFGILEFIS IAVGLVSIRG VDSGLYLGMN EKGELYGSEK LTQECVFREQ FEENWYNTYS SNLYKHVDTG RRYYVALNKD GTPREGTRTK RHQKFTHFLP RPVDPDKVPE LYKDILSQS
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/ml.
Storage: Lyophilized recombinant Human Fibroblast Growth Factor-9(rhFGF-9) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-9 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Fibroblast Growth Factor-9 (FGF-9) is a heparin binding growth factor that belongs to the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development.Recombinant Human Fibroblast Growth Factor-9(rhFGF-9) produced in E.coli is a single non-glycosylated polypeptide chain containing 208 amino acids. A fully biologically active molecule, rhFGF-9 has a molecular mass of 23.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.