Recombinant FGF-basic(145aa), Human
Catalogue Numbers: BK0053-10, BK0053-50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 16.4 kDa, observed by reducing SDS-PAGE.
Purity: > 95% as analyzed by SDS-PAGE.
Biological Activity: ED50 < 0.25 ng/mL, measured by the cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 × 10ˆ6 units/mg
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI
KLQLQAEER
G VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN
NYNTYRSRKY TSWYVALKR
T GQYKLGSKTG PGQKAILFLP MSAKS
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.
Storage: Lyophilized recombinant Human FGF-basic (145a.a.) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human FGF-basic (145a.a.) should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.Recombinant Human FGF-basic (145a.a.)produced in E.coli is a single non-glycosylated polypeptide chain containing 145 amino acids. A fully biologically active molecule, rhFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.