Recombinant GM-CSF (Sargramostim), Human (P.pastoris-expre Ssed)
Catalogue Numbers: BK0341-10, BK0341-50
Sizes: 10μg, 50μg
Source: P. pastoris
Molecular Weight: 26-32kDa, observed by SDS-PAGE.
Purity: > 95 % as analyzed by SDS-PAGE.
Biological Activity: ED50< 0.2ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 5x 10ˆ6 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against 10 mM PB, pH7.0.
AA Sequence: MAPARS PSPSTQPWEH VNAIQEALRL LNLSRDTAAE MNEt VEVISE MFDLQEPTCL QTRLELYKQG LRGSLTKLKG PLTMMASHYK QHCPPTPETS CATQIITFES FKENLKDFLL VIPFDCWEPV QE
Endotoxin: <1.0EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/ml.
Storage: Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds[2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities[4]. Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells[5].Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in P. pastoris a glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 26-32kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.