Recombinant HCoV-229E Nucleocapsid Protein
Sizes: 50μg, 500μg
Catalogue Numbers: PKSV030285-50, PKSV030285-500
Citations, Manuals and MSDS Available upon request.
Abbreviation: Nucleocapsid
Target Synonym: HCoV-229E Nucleocapsid Protein; HCoV-229E coronavirus NP Protein; HCoV-229E np Protein; HCoV-229E novel coronavirus Nucleoprotein Protein
UNIProt ID: P15130
Target Species: HCoV
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P15130
Background: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Sequence: Met1-Asn389
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: Please contact us for more information.
Calculated MW: 47.2 kDa
Observed MW: 54 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only