Recombinant Human 14-3-3 epsilon/YWHAE Protein (GST Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032016-10, PKSH032016-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: 14-3-3 epsilon; YWHAE
Target Synonym: 14-3-3E; HEL2; KCIP-1; MDCR; MDS
UNIProt ID: P62258
Research Areas: Signal Transduction; Neuroscience; Stem cells
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-GST
Accession: P62258
Background: 14-3-3 Protein Epsilon acts as an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 protein epsilon binds to a large number of partners, usually by recognition of a phophoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Sequence: Met 1-Gln255
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 56.0 kDa
Observed MW: 58 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only