Recombinant Human 2B4/CD244 protein (His tag)
Sizes: 20μg, 100μg
Catalogue Numbers: PDMH100145-20, PDMH100145-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: 2B4; CD244
Target Synonym: Natural killer cell receptor 2B4; NK cell activation-inducing ligand; NAIL; NKR2B4; h2B4; SLAM family member 4; SLAMF4; CD244; 2B4
UNIProt ID: Q9BZW8
Research Areas: immunology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: Q9BZW8
Background: The CD244 antigen; also known as 2B4; is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production; which is expressed on all NK cells; a subpopulation of T cells; monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor; human 2B4 can effectively activate and enhance NK cell– mediated cytotoxicity; induce secretion of IFN-γ and matrix metalloproteinases (MMPs); as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2; CD48; CD58; CD84; Ly-9; and SLAM; 2B4 (CD244) is expressed on all human NK cells; a subpopulation of T cells; basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity; induces secretion of IFN-gamma and matrix metalloproteinases; and NK cell invasiveness.
Sequence: Met1-Arg221
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 24.2 kDa
Observed MW: 40 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only