Recombinant Human ABCB5 Protein (Trx Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032096-10, PKSH032096-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ABCB5
Target Synonym: ATP-binding cassette sub-family B member 5; P-glycoprotein ABCB5; ABCB5 P-gp; ABCB5;
UNIProt ID: Q2M3G0
Research Areas: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-Trx
Accession: Q2M3G0
Background: ATP-binding cassette sub-family B member 5(ABCB5) is a plasma membrane-spanning protein. ABCB5 is principally expressed in physiological skin and human malignant melanoma. ABCB5 has been suggested to regulate skin progenitor cell fusion and mediate chemotherapeutic drug resistance in stem-like tumor cell subpopulations in human malignant melanoma. It is commonly over-expressed on circulating melanoma tumour cells. Furthermore, the ABCB5+ melanoma- initiating cells were demonstrated to express FLT1 (VEGFR1) receptor tyrosine kinase which was functionally required for efficient xenograft tumor formation, as demonstrated by shRNA knockdown experiments.
Sequence: Ile141-Val247
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 29.4 kDa
Observed MW: 30 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only