Recombinant Human ACADM/MCAD Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032032-10, PKSH032032-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ACADM; MCAD
Target Synonym: Medium-Chain Specific Acyl-CoA Dehydrogenase Mitochondrial; MCAD; ACADM
UNIProt ID: P11310
Research Areas: Signal Transduction; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P11310
Background: Medium-Chain Specific Acyl-CoA Dehydrogenase (ACADM) is a mitochondrial fatty acid beta-oxidation that belongs to the acyl-CoA dehydrogenase family. ACADM is a homotetramer enzyme that catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. ACADM is specific for acyl chain lengths of 4 to 16. It is essential for converting these particular fatty acids to energy, especially during fasting periods. Defects in ACADM cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death.
Sequence: Lys26-Asn421
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 0.1M NaCl, 20% Glycerol, pH 8.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 45.9 kDa
Observed MW: 42 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only