Recombinant Human AGO2/Argonaute 2/EIF2C2 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031271-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: AGO2; Argonaute 2; EIF2C2
Target Symptom: Argonaute 2; EIF2C2; Q10
Research Area: epigenetics and nuclear signal
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: N-His
Accession: NP_036286.2
Background: Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 is a regulator of dFMR1 expression and has an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.
Activity: Human AGO2 can bind Let-7a RNA and cleave target RNA (21nt).
Sequence: Met 1-Ala 859
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% glycerol, 2mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 99 kDa
Observed MW: 99 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only