Recombinant Human AKR1C3 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032055-10, PKSH032055-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: AKR1C3
Target Synonym: Aldo-Keto Reductase Family 1 Member C3; 17-Beta-Hydroxysteroid Dehydrogenase Type 5; 17-Beta-HSD 5; 3-Alpha-HSD Type II Brain; 3-Alpha-Hydroxysteroid Dehydrogenase Type 2; 3-Alpha-HSD Type 2; Chlordecone Reductase Homolog HAKRb; Dihydrodiol Dehydrogenase 3; DD-3; DD3; Dihydrodiol Dehydrogenase Type I; HA1753; Indanol Dehydrogenase; Prostaglandin F Synthase; Testosterone 17-Beta-Dehydrogenase 5; Trans-1; 2-Dihydrobenzene-1; 2-Diol Dehydrogenase; AKR1C3; DDH1; HSD17B5; KIAA0119; PGFS
UNIProt ID: P42330
Research Areas: Signal Transduction; Neuroscience; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P42330
Background: AKR1C3, is an enzyme which belongs to the aldo/keto reductase family. It is expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. AKR1C3 catalyzes the conversion of aldehydes and ketones to alcohols. It catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha, 11-beta-PGF2 to PGD2, which functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. It can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites.
Sequence: Met 1-Tyr323
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 37.8 kDa
Observed MW: 38 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only