Recombinant Human ALOX5AP/FLAP Protein (His Tag)
Size: 100μg
Catalogue Number: PKSH031059-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: ALOX5AP, FLAP
Target Synonym: FLAP
Research Areas: Signal Transduction, Cardiovascular, immunology,
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: C-His
Accession: NP_001620.2
Background: Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.
Sequence: Met 1-Pro 161
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, 10% glycerol, pH 7.4, 0.5% triton, 5mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 19 kDa
Observed MW: 19 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only