Recombinant Human Alpha-Synuclein/SNCA Protein (Active)
Size: 50μg
Catalogue Number: PKSH033771-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SNCA
Target Symptom: Alpha-Synuclein; Non-A Beta Component of AD Amyloid; Non-A4 Component of Amyloid Precursor; NACP; SNCA; NACP; PARK1
Research Area: Neuroscience
Target Species: Human
Expression Host: E.coli
Fusion Tag: None
UNIProt ID: P37840
Accession: P37840
Background: Alpha-Synuclein (SNCA) is a member of the Synuclein family. SNCA is expressed principally in brain but also expressed in low concentrations in all tissues except liver. SNCA interacts with UCHL1; Phospholipase D and histones. SNCA can include beta- and gamma-synuclein. In addition; SNCA is an important regulatory component of vesicular transport in neuronal cells. It has been suggested that SNCA is related to the pathogenesis of Parkinson' s Disease and neurodegenerative disorders. Defects in SNCA will lead to Dementia Lewy Body (DLB).
Activity: Recombinant Human alpha -Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1. Aggregation T1/2 time of 70 µM human alpha -Synuclein protein is 1.07h(0.5 mM SDS).
Sequence: Met1-Ala140
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS; PH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 14.46 kDa
Observed MW: 17 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only