Recombinant Human Arginase-1/ARG1 Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033453-10, PKSH033453-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Arginase-1; ARG1
Target Symptom: Arginase-1; Liver-type arginase; Type I arginase; ARG1
Research Area: Signal Transduction; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-6His
Accession: NP_000036.2
Background: Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.
Activity: Measured by the production of urea during the hydrolysis of arginine. The specific activity is 46411 pmol/min/µg.
Sequence: Met 1-Lys322
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mMNaCl, 20%Glycerol, 1mMDTT, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 35.6 kDa
Observed MW: 38 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only