Recombinant Human Arylsulfatase A/ARSA Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032093-10, PKSH032093-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Arylsulfatase A; ARSA
Target Synonym: Arylsulfatase A; ASA; Cerebroside-Sulfatase; ARSA
UNIProt ID: P15289
Research Areas: Signal Transduction; Tags & Cell Markers; Cell biology; Neuroscience; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P15289
Background: Arylsulfatase A (ARSA) is a lysosomal enzyme that breaks down Cerebroside 3-Sulfate, a major constituent of the myelin sheet, into Cerebroside and Sulfate. The ARSA deficiency results in Metachromatic Leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms. Recombinant Human ARSA corresponds to the ARSA mature peptide and has sulfatase activity.
Sequence: Arg19-Ala507
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 52.9 kDa
Observed MW: 55 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only