Recombinant Human ATG5 Protein
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032101-10, PKSH032101-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ATG5
Target Synonym: Autophagy protein 5; APG5-like; Apoptosis-specific protein; APG5L; ASP; ATG5;
UNIProt ID: Q9H1Y0
Research Areas: Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: None
Accession: Q9H1Y0
Background: ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.
Sequence: Met 1-Asp275
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 0.2M NaCl, 1mM DTT, 40%glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 32.4 kDa
Observed MW: 33 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only