Recombinant Human B3GAT3 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033257-10, PKSH033257-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: B3GAT3
Target Symptom: B3GAT3; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3; Beta-1; 3-glucuronyltransferase 3; Glucuronosyltransferase I; GlcAT-I; GlcUAT-I; Gal beta-1; 3-Gal-R glucuronyltransferase; ;
Research Area: Signal Transduction; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
UNIProt ID: O94766
Accession: O94766
Background: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is an enzyme that in humans is encoded by the B3GAT3 gene, belongs to the glycosyltransferase 43 family. B3GAT3 is involved in a number of biological processes such as catalyzing the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans, forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate, gGlycosaminoglycans biosynthesis, transfering a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1, 3-Gal-beta-1, 4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans.It also plays a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins , hows strict specificity for Gal-beta-1, 3-Gal-beta-1, 4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc and stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.
Sequence: Glu72-Val335
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaC, 2mM EDTA, 20% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 30.4 kDa
Observed MW: 31-34 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only