Recombinant Human B7-H1/PD-L1/CD274 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP00068-10, RP00068-20, RP00068-50, RP00068-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human B7-H1/PD-L1/CD274 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Phe19-Thr239) of human PD-L1/B7-H1 (Accession #NP_054862.1) fused with a 6×His tag at the C-terminus.
Alternate Names: B7-H, B7H1, PDL1, PD-L1, hPD-L1, PDCD1L1, PDCD1LG1, CD274, PDL1, B7H1, PD-L1, PDCD1L1, PDCD1LG1, B7-H, CD274 molecule
SWISS: Q9NZQ7
GeneID: 29126
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE; ≥95 % as determined by HPLC.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: This protein is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation.
Bio-Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human PD-L1 at 10 μg/mL (100 μL/well) can bind Recombinant Human PD-1 with a linear range of 0.25-1.02 μg/mL.|2.Measured by its binding ability in a functional ELISA.Immobilized PE anti-human CD274 (B7-H1, PD-L1) Antibody at 1μg/mL (25 μL/well) can bind Human CD274 with a linear range of 0.46-18ng/mL.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERT
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only