Recombinant Human B7-H1/PD-L1/CD274 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP00184-10, RP00184-20, RP00184-50, RP00184-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human B7-H1/PD-L1/CD274 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe19-Thr239) of human PD-L1/B7-H1 (Accession #NP_054862.1) fused with an Fc, 6×His tag at the C-terminus.
Alternate Names: B7-H, B7H1, PDL1, PD-L1, hPD-L1, PDCD1L1, PDCD1LG1, CD274, PDL1, B7H1, PD-L1, PDCD1L1, PDCD1LG1, B7-H, CD274 molecule
SWISS: Q9NZQ7
GeneID: 29126
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1). PD-L1/B7-H1 is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human PD-1 at 5 μg/mL (100 μL/well) can bind Recombinant Human PD-L1 with a linear range of 0.5-2.2 μg/mL.
Source: HEK293 cells
Tag: C-hFc&His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Antigen Sequence: FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERT
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only